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依次经过抽提、分步硫酸铵沉淀、二乙氨乙基纤维素（ＤＥＡＥＣｅｌｕｌｏｓｅ）柱层析，以及聚丙烯酰胺自然胶制备电泳（ＰｒｅｐａｒａｔｉｖｅＰＡＧＥ）等分离步骤，从耐热性较强的“白阳”大白菜品种的黄化种苗中，分离和纯化了与耐热性密切相关的一个葡萄糖磷酸变位酶（ＰＧＭ）同工酶。通过ＳＤＳＰＡＧＥ电泳，纯化的酶样品在２９ＫＤ处呈现一个显著条带。推测此酶为细胞质来源。我们还同时纯化了与耐热性无关的另一个细胞质ＰＧＭ同工酶。结果表明，它们只有自然电泳迁移率（电荷量）的不同，而没有明显分子大小的差别。在整个纯化过程中巯基保护试剂的存在对于ＰＧＭ酶的稳定性至关重要
Followed by extraction, step-by-step ammonium sulfate precipitation, diethylaminoethyl cellulose (DEAE Celeclose) column chromatography, and polyacrylamide gel preparation of natural electrophoresis (PreparativePAGE) and other separation steps, from the heat-resistant “ Baiyang ”cabbage varieties of yellow seedlings, isolated and purified and closely related to heat resistance of a glucose phosphate mutase (PGM) isoenzyme. Purified enzyme samples showed a significant band at 29 kD by SDS-PAGE. Presumably this enzyme is a cytoplasm source. We also simultaneously purified another cytoplasmic PGM isozyme not related to heat tolerance. The results showed that they only have different natural electrophoretic mobility (charge), but no obvious difference in molecular size. The presence of a thiol protecting reagent throughout the purification is crucial for the stability of the PGM enzyme