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目的：；探讨臭氧在糖尿病视网膜病变病理改变中的机制研究，为臭氧在糖尿病视网膜病变病理改变中的应用提供理论依据。方法：选取雄性健康成年（Sprague-Dawley，SD）糖尿病大鼠40只为研究对象，随机分成对照组和观察组，每组20只，对照组采用氧气灌肠，观察组采用臭氧灌肠，分析比较两组视网膜组织中细胞凋亡情况，分析比较两组大鼠视网膜中神经损伤情况，分析比较两组大鼠视网膜中内质网应激分子情况。结果：观察组视网膜组织中细胞凋亡数目明显高于对照组，两组之间差异显著，具有统计学意义（ P＜0.05）；观察组视网膜中神经损伤情况和视网膜中内质网应激分子情况明显高于对照组，两组之间差异显著，具有统计学意义（P＜0.05）。结论：臭氧能有效的控制大鼠视网膜中组织的缺氧情况，改善组织氧气的输送，延缓糖尿病视网膜病变的进展。“,”Objective: To investigate the mechanism of ozone in the pathological changes of diabetic retinopathy, and to provide theoretical basis for the application of ozone in the pathological changes of diabetic retinopathy. Methods: select healthy male adult male Sprague Dawley (SD) in diabetic rats 40 as the object of study randomly divided into the observation group and the control group, with 20 rats in each group, control group using oxygen enema, observation group was treated with ozone clysis, analysis of cell apoptosis in retinal tissue was compared between the two groups, analysis were compared between the two groups of rats retinal nerve injury. Analysis were compared between the two groups in the retina of rats in endoplasmic reticulum stress molecules. Results: observation group in retinal tissue of the number of apoptotic cells was significantly higher than that of the control group, there was a significant difference between the two groups, with statistical significance (P < 0.05); observation group in the retina nerve damage in the retina and endoplasmic reticulum stress molecules was significantly higher than that of the control group, there was a significant difference between the two groups, with statistical significance (P < 0.05). Conclusion: ozone can effectively control the hypoxia of the tissue in rat retina, improve the delivery of tissue oxygen and delay the progression of diabetic retinopathy.