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用11～12周龄的CFW系雄性小鼠,连续3天给予微量铝、铍、镉、钴、铜、氟、汞、铟、镧、镁、锰、钴、镍、铅、锑、钪、硒、钍、钛、锆、钇、釩等21种金属。此后每天3次共6次腹腔注射。注射量用对2-硫代-6-氧代间氮苯苦味的味觉阈值发生变化的最小有效量。注射金属的第3天给抗原,注射结束的第2天进行免疫反应研究。对照组仅注射蒸馏水。
The mice of CFW strain of 11-12 weeks old were given trace amounts of aluminum, beryllium, cadmium, cobalt, copper, fluorine, mercury, indium, lanthanum, magnesium, manganese, cobalt, nickel, lead, Selenium, thorium, titanium, zirconium, yttrium, vanadium and other 21 kinds of metal. After 3 times a day a total of 6 times intraperitoneal injection. Injection volume is the minimum effective amount that varies with the taste threshold of 2-thio-6-oxo-azinobenzene bitterness. On the third day after injection of the metal, the antigen was subjected to an immunological reaction study on the second day after the injection was completed. Control group only injected distilled water.