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目的:探讨脂联素、瘦素和肿瘤坏死因子(TNF)-α与酒精性脂肪肝严重程度的相关性。方法:45只Wistar大鼠分为对照、饮酒和甜菜碱组。酶比色法检测肝脏三酰甘油(TG)含量。酶联免疫吸附测定脂联素、瘦素和TNF-α。相关性采用Pearson相关和多元线性回归分析。结果:饮酒导致肝脏TG含量增高,血清和肝脏脂联素降低、瘦素增高。与饮酒组相比,甜菜碱组肝脏TG减少,血清、肝脏脂联素增高,血清、肝脏瘦素无差异。肝脏TNF-α水平在3组间无差异。血清脂联素与肝脏TG含量呈负相关(r=-0.605,P=0.000)。血清瘦素、肝脏脂联素和瘦素不是肝脏TG含量的独立影响因子。结论:血清脂联素是预测酒精性脂肪肝严重程度的重要指标,提高血清脂联素可降低肝脏TG含量,瘦素和TNF-α与酒精性脂肪肝无独立相关性。
Objective: To investigate the correlation between adiponectin, leptin, tumor necrosis factor (TNF) -α and the severity of alcoholic fatty liver. Methods: 45 Wistar rats were divided into control, alcohol consumption and betaine groups. Enzyme colorimetric assay for liver triglyceride (TG) content. Adiponectin, leptin and TNF-α were measured by ELISA. Correlation using Pearson correlation and multiple linear regression analysis. Results: Alcohol consumption led to increased liver TG content, serum and liver adiponectin decreased leptin increased. Compared with the drinking group, betaine liver TG decreased, serum, liver adiponectin increased, serum, liver leptin no difference. Liver TNF-α levels were not different among the three groups. Serum adiponectin and liver TG content was negatively correlated (r = -0.605, P = 0.000). Serum leptin, hepatic adiponectin, and leptin are not independent predictors of liver TG content. Conclusion: Serum adiponectin is an important index for predicting the severity of alcoholic fatty liver disease. Increasing serum adiponectin can reduce the content of TG in the liver. There is no independent correlation between leptin and TNF-α and alcoholic fatty liver.