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目的研究中性粒细胞在肝脏中的聚集、滞留机制及参与肝细胞损伤的作用.方法采用体外人肝细胞和中性粒细胞复合培养、用带有3μm孔径的碳素膜将肝细胞和中性粒细胞隔离及生物力学技术(微管吸吮系统)等方法,动态观察了烧伤病人(50%以上,Ⅱ°-Ⅲ)血清直接对肝细胞的损伤,在中性粒细胞参与下的损伤以及两细胞问粘附力的变化.结果①烧伤血清直接对肝细胞有损伤作用;②中性粒细胞参与可以加重损伤;③将中性粒细胞和肝细胞隔离后肝细胞损伤明显减轻;④肝细胞和中性粒细胞在烧伤血清作用下粘附力以2小时为最强,后随细胞损伤的加重渐减弱.结论中性粒细胞参与可加重肝细胞损伤,中性粒细胞和肝细胞间有粘附行为,并且粘附行为是加重肝细胞损伤的重要因索之一.
Objective To study the aggregation and retention mechanism of neutrophils in the liver and the role of participating in the injury of hepatocytes.Methods Human hepatocytes and neutrophils were cultured in vitro and cultured with carbon membrane with 3 μm pore size, (Microtubule sucking system) and other methods, dynamic observation of the burn patients (more than 50%, Ⅱ ° -Ⅲ) serum directly on the liver cell damage, with the involvement of neutrophils in the injury and The changes of adhesion between the two cells were observed.Results①The serum of burn patients had a direct damage on the hepatocytes.②The neutrophils involved could aggravate the injury.③The damage of hepatocytes was significantly reduced after the neutrophils and hepatocytes were isolated.④The liver The adhesion of cells and neutrophils to burn serum for 2 hours was the strongest, followed by the aggravation of cell injury.Conclusion The involvement of neutrophils can aggravate the damage of hepatocytes, the changes of neutrophils and hepatocytes Adhesion behavior, and adhesion behavior is one of the important factors aggravating hepatocellular injury.