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目的:研究ABCG2在胶质瘤血管形成过程中与VEGF、VEGFR和CD34的关系,探讨其在血管形成中的作用及对胶质瘤患者生存预后的影响。方法:采用脑胶质瘤的组织芯片技术,分析ABCG2、VEGF和VEGFR(flt-1)在胶质瘤中的表达率,根据CD34阳性的血管计数判定微血管密度(MVD);另用免疫荧光共聚焦检测ABCG2与CD34、VEGF的共表达;用COX回归模型分析ABCG2对胶质瘤患者预后影响。结果:ABCG2、VEGF、VEGFR(flt-1)和CD34阳性表达率随着胶质瘤恶性程度的增加而增高。ABCG2Ⅰ、Ⅱ级之间无统计学差异,其余各级别之间存在统计学差异(P<0.05);ABCG2与病理级别呈正相关;ABCG2表达水平与MVD显著相关,γ=0.540,P<0.001。ABCG2、VEGF和VEGFR(flt-1)均为阳性表达的肿瘤标本MVD平均值显著高于ABCG2阴性表达者,P<0.001。ABCG2与CD34、VEGF共表达于血管壁。COX回归模型证明ABCG2是胶质瘤患者预后的危险因素。结论:ABCG2阳性表达细胞具有向肿瘤血管细胞分化的潜能,对肿瘤血管研究重要意义,且ABCG2表达可作为考察胶质瘤患者预后的重要指标。
OBJECTIVE: To study the relationship between ABCG2 and VEGF, VEGFR and CD34 in the process of glioma angiogenesis and to explore the role of ABCG2 in angiogenesis and prognosis of glioma. Methods: The expression of ABCG2, VEGF and VEGFR (flt-1) in gliomas was analyzed by tissue microarray technique. The microvessel density (MVD) was determined according to the count of CD34 positive vessels. The co-expression of ABCG2, CD34 and VEGF was detected by FOC and the prognosis of glioma patients was analyzed by COX regression model. Results: The positive rate of ABCG2, VEGF, VEGFR (flt-1) and CD34 expression increased with the malignant degree of glioma. There was no significant difference between ABCG2 Ⅰ and Ⅱ levels, there was a significant difference between other levels (P <0.05). ABCG2 was positively correlated with pathological grade. ABCG2 expression was significantly correlated with MVD, γ = 0.540, P <0.001. The mean MVD of tumor specimens with positive expression of ABCG2, VEGF and VEGFR (flt-1) was significantly higher than that of ABCG2 negative expression (P <0.001). ABCG2 and CD34, VEGF co-expression in the vessel wall. COX regression model proved that ABCG2 is a risk factor for the prognosis of glioma patients. CONCLUSION: ABCG2 positive cells have the potential to differentiate into tumor vascular cells and have important significance for the study of tumor blood vessels. ABCG2 expression can be used as an important index to evaluate the prognosis of glioma patients.