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目的研究念珠菌性外阴阴道炎患者身体不同部位的白念珠菌基因型分布情况。方法采用PCR方法扩增白念珠菌包含Ⅰ类内含子的编码rRNA的25SrDNA区,根据扩增条带大小进行基因分型:A型(~450bp),B型(~840bp),C型(~450bp和~840bp)。结果60例念珠菌性外阴阴道炎患者中,54例由白念珠菌感染所致,其中表现为非复发性感染者39例,复发性感染者15例;同时从2个或2个以上部位分离到白念珠菌的病例共32例(32/54),其中非复发性念珠菌性外阴阴道炎19例(19/39),复发性念珠菌性外阴阴道炎13例(13/15)。从54例白念珠菌性外阴阴道炎患者的阴道、肛门、口腔部位共分离到92株白念珠菌,其中A型80株,B型8株,C型4株。同一患者不同部位基因型相同者24例(24/32),不同者8例(8/32)。结论在白念珠菌性外阴阴道炎中,主要以A型白念珠菌为主,B型、C型所占比例较少,且多分离自非阴道部位。在复发性念珠菌性外阴阴道炎患者中,身体其他部位念珠菌的定植率高于非复发性念珠菌性外阴阴道炎,肠道菌库理论在念珠菌性外阴阴道炎的反复发作中,具有一定的作用。
Objective To study the distribution of Candida albicans genotypes in different parts of the body of patients with candidal vulvovaginitis. Methods The 25S rDNA region of Candida albicans containing type Ⅰ intron was amplified by PCR and genotyped according to the size of the amplified bands: type A (~ 450bp), type B (~ 840bp), type C ( ~ 450 bp and ~ 840 bp). Results Of the 60 cases of candida vulgaris, 54 cases were caused by Candida albicans infection, of which 39 cases were non-recurrent infection and 15 cases were recurrent infection; at the same time, 2 or more sites were isolated Candida albicans to 32 cases (32/54), of which 19 cases of non-recurrent candidal vulvovaginitis (19/39), recurrent candidal vulvovaginitis in 13 cases (13/15). 92 Candida albicans strains were isolated from 54 cases of Candida albicans vulvovaginitis in vagina, anus and oral cavity, of which 80 were type A, 8 were type B and 4 were type C. The same patient in different parts of the same genotype in 24 cases (24/32), different in 8 cases (8/32). Conclusions Candida albicans is the main type in Candida albicans vaginitis, while type B and C are less frequently accounted for, and are mostly isolated from non-vaginal parts. In patients with recurrent candidal vulvovaginitis, Candida colonization in other parts of the body is higher than non-recurrent candidal vulvovaginitis, gut bacteria library theory in recurrent candida vulvovaginitis, with A certain role.