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目的:尽早发现登革热与基孔肯雅热病例,及时采取有效防控措施。方法:对2011年6月20日-2011年8月31日东莞市应急监测病例及疫情监测病例血清样品,采用荧光PCR检测登革热与基孔肯雅热病毒核酸,登革热核酸检测阴性者,再用ELISA法检测登革热病毒IgM抗体。结果:共收集及检测248份合格血清样品。其中,应急监测病例血清234份,检测结果均为阴性;疫情监测病例血清14份,检出2份为登革热IgM抗体阳性,1份为登革热核酸阳性,为IV型;确诊2例为登革热输入性病例。结论:通过实验室应急监测,发现了登革热病例,及时控制了疫情的传播扩散。
OBJECTIVE: To detect dengue fever and chikungunya as soon as possible and to take effective prevention and control measures in time. Methods: From June 20, 2011 to August 31, 2011, the serum samples of emergency surveillance cases and epidemic surveillance cases in Dongguan were detected by fluorescence PCR. The dengue and chikungunya virus nucleic acids were detected by PCR. Detection of dengue virus IgM by ELISA. Results: A total of 248 qualified serum samples were collected and tested. Among them, 234 serum samples were obtained from emergency monitoring and the results were negative. In the 14 cases of epidemic situation monitoring, 2 were positive for dengue IgM antibody, 1 were positive for dengue nucleic acid and were type IV; 2 were diagnosed as dengue fever Case. Conclusion: Through the laboratory emergency monitoring, we found dengue fever cases and controlled the spread of the epidemic in time.