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Objective:To explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1(ET-1) in vitro.Methods:A cell culture model,[~3H]-thymidine([3~H]-TdR) incorporation test and confocal microscope were used to observe proliferation and intracellular free calcium concentration([Ca~(2+)]_i) of rabbit PASMC induced by ET-1 in vitro.Results:The value of [3~H]-TdR incorporation in ET-1 group was increased 1.468 times higher than that in control group.Iptakalim at the concentration of 10~(-7)mol/L,10~(-6) mol/L,10~(-5) mol/L lowered [~3H]-TdR incorporation by (19.8±4.6)%,(41.2±9.5)%,(54.7±10.1)%,respectively,compared with the value of the cells treated with ET-1(P<0.01); The intracellular fluorescence intensity of PASMC in ET-1 group was increased from 73.70±10.12 to 143.84±28.23,significantly higher than that in control group(P<0.01);whereas with Iptakalim,the fluorescence intensity(FI) was only increased from 74.30±10.20 to 86.03±9.82,significantly lower than that in ET-1 group(P<0.01).Conclusion:Iptakalim inhibited proliferation of PASMC and decreased intracellular free calcium concentration of cultured rabbit PASMC induced by ET-1.
Objective: To explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1 (ET-1) in vitro. Methods: A cell culture model, [~ 3H] thymidine ([3-H] -TdR) incorporation test and confocal microscope were used to observe proliferation and intracellular free calcium concentration ([Ca ~ (2 +)] _i) of rabbit PASMC induced by ET-1 in vitro. Results: The value of [3 ~ H] -TdR incorporation in ET-1 group was increased by 1.468 times higher than that in control group. Iptakalim at the concentration of 10 ~ (-7) mol / L, 10 ~ (-6) mol / L , Respectively, compared with the value of the cells treated with 10 -5 mol / L lowered [~ 3H] -TdR incorporation by (19.8 ± 4.6)%, (41.2 ± 9.5)%, (54.7 ± 10.1)%, with intracellular fluorescence intensity of PASMC in ET-1 group was increased from 73.70 ± 10.12 to 143.84 ± 28.23, significantly higher than that in control group (P <0.01); while with Iptakalim, the fluorescence The intensity (FI) was only increased from 74.30 ± 10.20 to 86.03 ± 9.82, significantly lower than that in ET-1 group (P <0.01) .Conclusion: Iptakalim inhibited proliferation of PASMC and decreased intracellular free calcium concentration of cultured rabbit PASMC induced by ET-1.