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目的：研究全反式维甲酸对血管平滑肌细胞增殖，ＤＮＡ合成和转化生长因子－β（ＴＧＦ－β）基因表达的影响。方法：采用血管平滑肌细胞培养，细胞计数，３Ｈ－ＴｄＲ参入，斑点杂交和图像分析方法。结果：全反式维甲酸（Ａ－ＴＲＡ，０．０１，０．１，１μｍｏｌ·Ｌ－１）明显抑制胎牛血清（ＦＣＳ）促进的血管平滑肌细胞增殖和ＤＮＡ合成，其最大抑制率分别是２２．４％和８７．１％；全反式维甲酸明显抑制ＦＣＳ促进的血管平滑肌细胞ＴＧＦ－βｍＲＮＡ表达。结论：ＡＴＲＡ具有抗血管平滑肌细胞增殖作用，其机制与抑制ＴＧＦ－β基因表达有关。
Objective: To investigate the effects of all-trans retinoic acid on the proliferation, DNA synthesis and expression of TGF-β in vascular smooth muscle cells. Methods: Vascular smooth muscle cell culture, cell counting, 3H-TdR incorporation, dot blot hybridization and image analysis were used. Results: All-trans retinoic acid (A-TRA, 0.01,0.1,1μmol·L-1) significantly inhibited the proliferation and DNA synthesis of vascular smooth muscle cells stimulated by fetal calf serum (FCS). The maximum inhibitory rates were 22.4% and 87.1% respectively. All-trans retinoic acid significantly inhibited the expression of TGF-βmRNA in FCS-stimulated vascular smooth muscle cells. CONCLUSION: ATRA can inhibit the proliferation of vascular smooth muscle cells and its mechanism is related to the inhibition of TGF-β gene expression.