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[Objective] To explore protective effects of Lycium barbarum L. polysaccharides component-II (LBP-II) on paraoxon (PO)-induced vascular endothelium dysfunction, and analyze its potential mechanism. [Methods]The human umbilical veins endothelial cells (HUVECs) were exposed to medium containing 3.63 μmol/L PO, and 10 μmol/L LBP-II was used to inhibit damage effect. [Results] Exposure to PO markedly reduced the level of nitric oxide (NO) and the activity of superoxide dismutase (SOD), significantly increased the level of malondialdehyde (MDA) in HUVECs cultured medium. However, treatment of LBP-II markedly ameliorated the morphology of HUVECs impaired by PO, while significantly lessened the HUVECs monolayer permeability and cell apoptosis. It was also examined that exposure of LBP-II markedly raised the decreased level of SOD and NO induced by PO, attenuated the increased activity of MDA induced by PO in HUVECs cultured medium. In addition, treatment of 1.0 μmol/L hydrogen peroxide (HA) showed a similar effect to LBP-II on PO-induced damnification. [Conclusion] LBP-II could protect the HUVECs against the PO-induced damnification, beneficial effects of which might be concerned with its antioxidation due to inhibiting the decreased activity of PON1.
[Objective] To explore protective effects of Lycium barbarum L. polysaccharides component-II (LBP-II) on paraoxon (PO) -induced vascular endothelium dysfunction, and analyze its potential mechanism. [Methods] The human umbilical veins endothelial cells (HUVECs) were exposed to medium containing 3.63 μmol / L PO, and 10 μmol / L LBP-II was used to inhibit damage effect. [Results] Exposure to PO markedly reduced the level of nitric oxide (NO) and the activity of superoxide dismutase (SOD However, the treatment of LBP-II markedly ameliorated the morphology of HUVECs impaired by PO, while significantly lessened the HUVECs monolayer permeability and cell apoptosis. It was also examined that exposure of LBP-II markedly raised the decreased level of SOD and NO induced by PO, attenuated the increased activity of MDA induced by PO in HUVECs cultured medium. In addition, treatment of 1.0 μmol / L hydrogen peroxide (HA ) LBP-II could protect the HUVECs against the PO-induced damnification, beneficial effects of which might be concerned with its antioxidation due to the decreased activity of PON1 .