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目的:探讨S100A11在结肠癌和正常肠粘膜组织中的表达及其与患者临床特征的的关系。方法:采用RT-PCR、WesternBlotting技术,检测S100A11在24例结肠癌及正常肠粘膜中的表达,并分析S100A11与患者年龄,性别,临床病理分型之间的关系。结果:S100A11mRNA在结肠癌组织的表达量(0.944+0.032)高于正常肠粘膜组织中的表达量(0.828+0.079),两组比较差异有统计学意义(p<0.05)。S100A11蛋白在结肠癌组织中的表达量(0.951+0.02)高于在正常肠粘膜组织中的表达量(0.860+0.05),两组比较差异有统计学意义(p<0.05)。但与患者临床特征之间比较差异无统计学意义(p>0.05)。结论:S100A11在结肠癌组织中表达量高于正常肠粘膜组织,提示其与结肠癌的发生和发展有关.是判断结肠癌生物学行为的有价值的参考指标。
Objective: To investigate the expression of S100A11 in colon cancer and normal intestinal mucosa and its relationship with clinical features. Methods: The expression of S100A11 in 24 cases of colon carcinoma and normal intestinal mucosa was detected by RT-PCR and Western Blotting. The relationship between S100A11 and age, gender and clinicopathological type was analyzed. Results: The expression of S100A11 mRNA in colon cancer tissues (0.944 + 0.032) was higher than that in normal intestinal mucosa tissues (0.828 + 0.079). There was significant difference between the two groups (p <0.05). The expression of S100A11 protein in colon cancer tissues (0.951 + 0.02) was higher than that in normal mucosa tissues (0.860 + 0.05). The difference between the two groups was statistically significant (p <0.05). However, there was no significant difference with the clinical features of patients (p> 0.05). Conclusion: The expression level of S100A11 in colon cancer tissues is higher than that in normal intestinal mucosa, suggesting that S100A11 is associated with the occurrence and development of colon cancer, which is a valuable reference to judge the biological behavior of colon cancer.