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目的探讨不同血小板制剂对细胞体外增殖活力的影响,并对其作用机制进行初步研究。方法制备不同血小板制剂:新鲜机采血小板、冰冻机采血小板、富血小板凝胶上清、乏血小板血浆等,以1640培养基为对照,与MM.1S细胞(多发性骨髓瘤细胞)在相同的培养条件下进行共培养,用流式细胞仪对培养后的细胞进行细胞计数,观察细胞的增殖情况。结果 1)单独的血小板及乏血小板血浆均对MM.1S细胞有促增殖作用,且促增殖效果与样品浓度成正相关。2)新鲜机采血小板对MM.1S细胞促增殖作用在各实验组中最高,新鲜机采凝胶上清和乏血小板血浆次之;二甲基亚砜对细胞增殖有抑制作用;凝血酶对细胞增殖无明显影响。3)血小板经过冰冻处理后,机采冰冻血小板与冰冻凝胶上清对MM.1S细胞促增殖作用较新鲜机采血小板和凝胶上清减弱。但机采冰冻血小板与冰冻凝胶上清对MM.1S细胞的促增殖作用存在剂量效应,在稀释浓度40-80倍左右时,具有相对较强的促增殖影响力。结论不同血小板制剂对MM.1S细胞的促增殖作用各有不同,此研究将为制备血小板凝胶选择原辅材料提供实验依据和参考。
Objective To investigate the effects of different platelet preparations on cell proliferation in vitro and its mechanism of action. Methods Different platelet preparations were prepared by fresh platelet-collecting, frozen platelet-collecting, platelet-rich serum and platelet-poor plasma. The cells were treated with 1640 medium as the same as MM.1S cells (multiple myeloma cells) Cultures were performed under culture conditions, and the number of cultured cells was counted by flow cytometry to observe the proliferation of cells. Results 1) Both platelets and platelet-poor plasma had an effect of promoting proliferation of MM.1S cells, and the effect of promoting proliferation was positively correlated with the sample concentration. 2) Fresh platelets on MM.1S cells promote the proliferation of the highest in each experimental group, followed by fresh machine-picked gel supernatant and platelet-free plasma; dimethyl sulfoxide inhibition of cell proliferation; thrombin on cells No significant effect on proliferation. 3) Platelets after freezing treatment, machine-made frozen platelets and frozen gel supernatant of MM.1S cells promote proliferation than fresh machine platelets and gel supernatant weakened. However, there was a dose-effect on the proliferation of MM.1S cells by using frozen platelets and frozen gel supernatant, and had a relatively strong effect of promoting proliferation when the dilution concentration was about 40-80 times. Conclusion Different platelet preparations have different effects on promoting the proliferation of MM.1S cells. This study will provide experimental evidence and reference for the preparation of platelet gels.