羟喜树碱对人肾上腺皮质癌SW-13细胞迁移的影响

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目的:观察羟喜树碱对人肾上腺皮质癌SW-13细胞迁移的影响.方法:体外培养人肾上腺皮质癌SW-13细胞,取对数生长期细胞(同一代)进行实验,随机分为羟喜树碱干预组和对照组,干预组加入0.8ug/ml羟喜树碱.细胞划痕试验观察SW-13细胞的体外迁移力,分别在0h、48h拍照观察,应用Image pro plus 6软件处理分析结果.结果:1、光镜下可见羟喜树碱干预组SW-13细胞未迁移面积大于对照组;2、羟喜树碱干预组SW-13细胞的未迁移率大于对照组(P=0.000).结论:羟喜树碱有可能降低人肾上腺皮质癌SW-13细胞侵袭、迁移的能力.“,”Objective To investigate the effects of HCPT on the migration of human adrenoeortical carcinoma cel line SW-13.Method SW-13 cel were culutured and the same cels were studied,which were randomly divided into there groops:The 0.8ug/mlHCPT intervention group and negative control group. The migration ability of SW-13 was measured by non-migration area.The size of the non-migration area was photographed at 0,48h and analyzed with the Image pro plus 6 software.Results At 48 hour, the non-migration area percentage in the experimental group were larger than negative Control .Conlusions HCPT could inhibit the migration ability of SW-13 cels. Objective To investigate the effects of HCPT on the migration of human adrenoeortical carcinoma cel line SW-13.Method SW-13 cel were culutured and the same cels were studied,which were randomly divided into there groops:The 0.8ug/mlHCPT intervention group and negative control group. The migration ability of SW-13 was measured by non-migration area.The size of the non-migration area was photographed at 0,48h and analyzed with the Image pro plus 6 software.Results At 48 hour, the non-migration area percentage in the experimental group were larger than negative Control .Conlusions HCPT could inhibit the migration ability of SW-13 cels.
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