论文部分内容阅读
目的建立测定K562/G01细胞株(imatinib-resistant K562 cells,耐伊马替尼K562细胞株)内伊马替尼浓度的方法,探讨甲基莲心碱对其细胞内STI571转运的影响。方法采用反相高效液相色谱法(reversedphase high-performance liquid chromatographic,RP-HPLC)直接测定药物浓度。色谱柱为:Gemini C18(250 mm×4.6 mm,5μm);流动相为:0.02 mol L-1KH2PO4-CH3CN=65:35;流速:1.0 mL min-1;检测波长:265 nm。结果采用外标法以峰面积定量,以色谱峰面积对STI571浓度进行线性回归,得回归方程:A=83.08C-7.975,r=0.999 4。线性范围49.51～3 076.92 ng mL-1,相对回收率分别>95%,日内日间RSD均<10%。结论该方法准确、快速、操作简便,可用于细胞内药物浓度的测定与研究。结果显示甲基莲心碱能影响细胞内STI571的转运。
Objective To establish a method for determining imatinib concentration in imatinib-resistant K562 cells (imatinib-resistant K562 cells) and investigate the effect of neferine on its intracellular STI571 transport. Methods The drug concentration was determined directly by reversed-phase high-performance liquid chromatography (RP-HPLC). The column was Gemini C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was 0.02 mol L -1 KH 2 PO 4-CH 3 CN = 65:35, the flow rate was 1.0 mL min -1 and the detection wavelength was 265 nm. Results The external standard method was used to quantify the peak area, and the linear regression of the peak area of STI571 was performed. The regression equation was: A = 83.08C-7.975, r = 0.999 4. The linear range was 49.51 ~ 3 076.92 ng mL-1. The relative recoveries were> 95% respectively. The intra-day and inter-day RSD were both <10%. Conclusion The method is accurate, rapid and easy to operate. It can be used in the determination and study of intracellular drug concentration. The results show that neferine can affect intracellular STI571 transport.