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目的 探讨缺氧对胎盘绒毛分泌肿瘤坏死因子 - α(TNF- α)和转化生长因子 - β1 (TGF- β1 )的影响。 方法 7例正常足月胎盘绒毛分别在常氧和低氧 (5 % O2 )条件下培养 ,采用 EL ISA方法测定胎盘绒毛培养液中 TNF-α和 TGF-β1 水平。 结果 低氧条件下培养 1、4、12、2 4h胎盘绒毛分泌TNF- α的量分别为 (6 9.5± 13.1、12 8.7± 16 .5、2 41.4± 90 .5、175 .3± 93.9) pg/克湿重 ,显著高于常氧条件下培养 ,分别为 (5 2 .4± 10 .7、99.3± 16 .1、147.9± 2 0 .3、116 .4± 2 1.2 ) pg/克湿重 ,但分泌 TGF-β1 的水平二者无显著性差异。 结论 胎盘缺氧能大量产生某种细胞因子 ,可能参与妊高征的病理发生过程。
Objective To investigate the effect of hypoxia on the secretion of tumor necrosis factor - α (TNF - α) and transforming growth factor - β1 (TGF - β1) in placental. Methods Totally 7 cases of normal term placental villi were cultured under normoxia and hypoxia (5% O2) respectively. The levels of TNF-α and TGF-β1 in placental villi were determined by ELISA. Results The amount of TNF-α secreted by placenta of 1, 4, 12, and 24 hours after hypoxia were (6 9.5 ± 13.1,12 8.7 ± 16.5,2 41.4 ± 90.5,175.3 ± 93.9) pg / g wet weight, significantly higher than that under normoxic conditions, and were (52.4 ± 10.7,99.3 ± 16.1,147.9 ± 2.03,116.4 ± 2.2) pg / g Wet weight, but the level of secretion of TGF-β1 no significant difference between the two. Conclusion Placental hypoxia can produce a large number of cytokines, which may be involved in the pathogenesis of PIH.