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AIM:To study the relationship between expression of platelet-derived growth factor-BB (PDGF-BB) and fibrogenesis inchronic hepatitis B.METHODS:Hepatic tissues from 43 patients with chronichepatitis B were embedded in paraffin.The sections werestained with HE and picric acid-sirius red to determineinflammatory activity and fibrosis stages.PDGF-BB expressionwas detected by immunohistochemistry and assessedsemiquantatively.Levels of serum hyaluronic acid (HA),pro-collagen Ⅲ(PCⅢ),collagen Ⅳ(Ⅳ-C) and laminin (LN)were examined by radioimmunoassay (RIA).RESULTS:The expression level of PDGF-BB was found tobe positively correlated with inflammatory activity,fbrosisstage and grade of histological findings (τ=0.58,0.55,0.55,P<0.01).The positive correlation was also observedbetween tissue level of PDGF-BB expression and contentsof HA,PC≡Ⅲ,Ⅳ-C and LN in the circulation (r=-0.52,0.32,0.40,0.33,P<0.05).CONCLUSION:PDGF-BB may play some role in thedevelopment and progression of liver fibrosis.
AIM: To study the relationship between expression of platelet-derived growth factor-BB (PDGF-BB) and fibrogenesis inchronic hepatitis B. METHODS: Hepatic tissues from 43 patients with chronic hepatitis B were embedded in paraffin. The sections were stained with HE and picric acid -sirius red to determineinflammatory activity and fibrosis stages. PDGF-BB expression was detected by immunohistochemistry and assessedsequilyquantly. Levels of serum hyaluronic acid (HA), pro-collagenⅢ (PCⅢ), collagenⅣ (Ⅳ-C) and laminin The results were examined by radioimmunoassay (RIA) .RESULTS: The expression level of PDGF-BB was found to positively positively with inflammatory activity, fbrosisstage and grade of histological findings (τ = 0.58,0.55,0.55, P <0.01). The positive correlation was also Observations of tissue level of PDGF-BB expression and contents of HA, PC≡III, IV-C and LN in the circulation (r = -0.52,0.32,0.40,0.33, P <0.05) .CONCLUSION: PDGF-BB may play some role in the development and progression of liver fib rosis.