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目的 :探讨新的凋亡相关基因 Survivin蛋白在宫颈癌中的表达及其与肿瘤细胞凋亡的关系。方法 :采用免疫组织化学 S- P法和 DNA原位末端标记 TUNEL法分别测定45例宫颈癌组织及 1 0例正常宫颈组织中 Survivin蛋白的表达和细胞凋亡。结果 :Survivin蛋白在宫颈癌组织中的阳性表达率为 68.89% ,与病理分级、临床分期和淋巴结转移密切相关 ( P<0 .0 5 ) ,而正常宫颈组织中无阳性表达 ;宫颈癌组织及正常宫颈组织中细胞凋亡指数( AI)分别为 7.5 5± 2 .35、5 .64± 1 .81 ,其差异有显著意义 ( P<0 .0 5 ) ;Survivin蛋白的表达与细胞凋亡指数呈负相关 ( r=- 0 .5 4 1 ,P<0 .0 1 )。结论 :Survivin蛋白的异常表达而引起的细胞凋亡抑制或逃避 ,在宫颈癌的发生、发展中起一定的作用 ;联合检测 Survivin蛋白和凋亡指数 ,有助于对肿瘤细胞的分化程度作出正确评价 ,以指导临床治疗及估计预后
Objective: To investigate the expression of survivin protein in cervical cancer and its relationship with tumor cell apoptosis. Methods: Survivin protein expression and apoptosis in 45 cervical cancer tissues and 10 normal cervical tissues were detected by immunohistochemical S-P method and DNA in situ TUNEL method. Results: The positive rate of Survivin protein in cervical cancer was 68.89%, which was closely related to the pathological grade, clinical stage and lymph node metastasis (P <0.05), but not in normal cervical tissues. The apoptosis index (AI) in normal cervical tissue was 7.5 5 ± 2 .35 and 5 .64 ± 1 .81 respectively, with significant difference (P <0.05). The expression of Survivin protein and apoptosis The index was negatively correlated (r = - 0 .5 4 1, P <0. 01). Conclusion: Inhibition or escape of apoptosis induced by the abnormal expression of Survivin protein may play an important role in the occurrence and development of cervical cancer. Combined detection of Survivin protein and apoptosis index will help to make the differentiation degree of tumor cells correct Evaluation, to guide the clinical treatment and prognosis