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中国汞矿资源丰富,其中贵州汞矿矿床云集,研究表明该地区汞矿一线工人及当地居民头发中的汞含量均远高于正常人群,这也说明当地居民长期处于高汞暴露状态。尿液中8-羟基-2′-脱氧鸟苷(8-OHdG)常常被用作活性氧自由基引起 DNA 氧化损伤的生物标志物。8-OHdG 的测定方法主要有高效液相色谱-电化学检测法(HPLC-ECD)、单克隆抗体酶联免疫法(ELISA)等。与其他方法相比,HPLC-ECD 具有灵敏度高、准确和直观等特点,近几
China has abundant mercury deposits and mercury deposits in Guizhou Province. Research shows that mercury content in hair of frontline mercury workers and local residents in the region is much higher than that of the normal population, indicating that the local residents are exposed to high levels of mercury for a long time. 8-hydroxy-2’-deoxyguanosine (8-OHdG) in the urine is often used as a biomarker of reactive oxygen species causing DNA oxidative damage. The main determination methods of 8-OHdG are high performance liquid chromatography-electrochemical detection (HPLC-ECD) and monoclonal antibody enzyme-linked immunosorbent assay (ELISA). Compared with other methods, HPLC-ECD has the characteristics of high sensitivity, accuracy and visualization, the recent