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采用闭塞大鼠四条动脉的全脑缺血模型，观察缺血３０ｍｉｎ及再灌注不同时期某些脑区中ＧＳＨ含量及ＧＳＨ－Ｐｘ活性的变化。结果显示，与对照组相比，缺血３０ｍｉｎ后ＧＳＨ、ＧＳＨ－Ｐｘ均有下降，随再灌期延长，ＧＳＨ含量逐渐回升，至再灌注４８ｈ恢复至原有水平；而ＧＳＨ－Ｐｘ活性在再灌注初期（２ｈ）有所回升，但至再灌注２４ｈ再度出现下降。这种变化趋势，在大脑皮层和海马中表现显著，而在丘脑和下丘脑中差异不显著或幅度较小。表明大脑皮层和海马是缺血神经元选择性易损区。Ｐ＜０．０１（与对照组相比）ｎ＝６由表１可知，与对照组相比，缺血３０ｍｉｎ时，ＧＳＨ、ＧＳＨ－Ｐｘ显著降低（Ｐ＜０．０１）。缺血３０ｍｉｎ后再灌注期间，随时间延长，ＧＳＨ逐步回升，至４８ｈ已基本恢复到对照组水平。ＧＳＨ－Ｐｘ在再灌注初期（２ｈ）略有回升，至２４ｈ又进一步降低，甚至低于缺血３０ｍｉｎ时；至４８ｈ有所增高，但显著低于对照组（Ｐ＜０．０１）。２．２缺血和再灌注时丘脑和下丘脑ＧＳＨ、ＧＳＨ－Ｐｘ变化由表２可知，与对照组相比，缺血３０ｍｉｎ时，ＧＳＨ含量有所下降，并随再灌注时间延长而升高，至４８ｈ己恢复原有水平，各组间均无显著差异。缺血３０ｍｉｎ表２缺血和?
The model of global cerebral ischemia in four rats occluded rats was used to observe the changes of GSH and GSH-Px activities in some brain regions at different time points after ischemia for 30 min and reperfusion. The results showed that compared with the control group, GSH and GSH-Px decreased at 30min after ischemia, GSH content gradually increased with the prolongation of reperfusion, returned to the original level 48h after reperfusion, while the activity of GSH-Px In the early period of perfusion (2h), it rebounded, but decreased again 24h after reperfusion. This trend of change, in the cerebral cortex and hippocampus significantly, while in the thalamus and hypothalamus, no significant difference or less. This suggests that the cerebral cortex and hippocampus are selective areas of vulnerability to neuronal ischemia. P <0.01 compared with control group n = 6 As can be seen from Table 1, GSH and GSH-Px were significantly decreased at 30 min after ischemia (P <0.01). During the reperfusion period after 30min of ischemia, GSH gradually rose with the passage of time and returned to the level of control group at 48h. GSH-Px increased slightly in the early stage of reperfusion (2h), further decreased to 24h, even lower than 30min after ischemia; increased to 48h, but significantly lower than that of the control group (P <0.01). 2.2 Ischemia and reperfusion of thalamus and hypothalamus GSH, GSH-Px changes seen from Table 2, compared with the control group, 30min ischemia, GSH levels decreased, and increased with the reperfusion time increased , To 48h has returned to its original level, no significant differences between the groups. Ischemia 30min Table 2 Ischemia and?