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建立了固相萃取-超高效液相色谱法快速检测新疆孕马尿中的3种主要结合雌激素——雌酮硫酸钠、马烯雌酮硫酸钠和17α-双氢马烯雌酮硫酸钠。采用大孔树脂固相萃取柱(SPE)富集孕马尿中的结合雌激素,以UPLC-PDA检测。采用C_(18)色谱柱,以0.025 mol/L磷酸二氢钾溶液-乙腈(77:23)为流动相,检测波长215 nm。3种结合雌激素分别在0.6～6.0、0.3～3.0和0.2～2 0μg/ml浓度范围内线性关系良好。平均回收率分别为97.0%、96.4%和98.3%,RSD分别为0.9%、1.7%和1.6%。
A rapid solid phase extraction coupled with ultra performance liquid chromatography (HPLC) was developed for the rapid determination of three major estrogen-estrone sulphate, equol estrone sulphate and 17α-dihydroequinate sodium sulphate . Macroporous resin SPE column was used to enrich the conjugated estrogen in pregnant and mare urine and detected by UPLC-PDA. The mobile phase was C_ (18) column and mobile phase was 0.025 mol / L potassium dihydrogen phosphate - acetonitrile (77:23). The detection wavelength was 215 nm. The three kinds of conjugated estrogens showed good linearity in the range of 0.6 ~ 6.0, 0.3 ~ 3.0 and 0.2 ~ 20μg / ml, respectively. The average recoveries were 97.0%, 96.4% and 98.3%, respectively, with RSDs of 0.9%, 1.7% and 1.6%, respectively.