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目的:探讨黄芪甲苷对人外周血内皮祖细胞的保护作用。方法:用密度梯度离心法获取单个核细胞(MNCs),培养7天后,收集贴壁细胞,采用多波长激光共聚焦显微镜(laserscanningeonfoealmicroscope,LSCM)鉴定后将细胞随机分成:(l)对照组;(2)辛伐他汀对照组;(3)黄芪甲苷组。24h后采用流式细胞仪检测EPCs的增殖周期;MTT比色法、黏附能力实验检测内皮祖细胞(En-dothelialprogenitoreells,EPCs)的增殖能力、黏附能力。结果:黄芪甲苷在10-1mmol/L～10-10mmol/L的浓度范围中均有效,在黄芪甲苷10-9mmol/L浓度时,EPCs的增殖力最强,黄芪甲苷能明显增强EPCs的黏附、增殖能力。结论:黄芪甲苷能够增强EPCs的生物学功能,对EPCs有保护作用。
Objective: To investigate the protective effect of Astragaloside IV on human peripheral blood endothelial progenitor cells. Methods: Mononuclear cells (MNCs) were obtained by density gradient centrifugation. After culturing for 7 days, adherent cells were collected and identified by laserscanningeonfoeal microscopy (LSCM). The cells were randomly divided into: (1) control group; 2) Simvastatin control group; (3) Astragaloside group. The proliferation cycle and EPCs proliferation of endothelial progenitor cells (EPCs) were detected by MTT assay and adhesion assay. Results: Astragaloside was effective in the concentration range of 10-1mmol / L ~ 10-10mmol / L. When the concentration of Astragaloside 10-9mmol / L, the proliferation of EPCs was the strongest, Astragaloside Ⅳ could significantly enhance the EPCs The adhesion, proliferation ability. Conclusion: Astragaloside IV can enhance the biological function of EPCs and protect EPCs.