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ｐ２７蛋白是细胞周期素依赖性激酶（Ｃｄｋ）抑制蛋白家族中的一种，主要对外部促进或抑制细胞增殖的信号起反应。本研究应用流式细胞仪（ＦＣＭ）双标记的方法观察血管紧张素Ⅱ（ＡｎｇⅡ）、血管加压素（ＡＶＰ）和血小板源生长因子（ＰＤＧＦ）对血管平滑肌细胞（ＶＳＭＣｓ）细胞周期百分比和ｐ２７蛋白表达量的影响。静止状态培养的ＶＳＭＣｓ加入ＡｎｇⅡ，ＡＶＰ，ＰＤＧＦＢＢ后，在不同时间收集细胞，用碘化丙啶（ＰＩ）标记细胞ＤＮＡ，以确定细胞所处的周期。用ｐ２７蛋白的单抗和标记了ＦＩＴＣ的二抗标记细胞，通过流式细胞仪测定被激发出的荧光量来确定细胞ｐ２７蛋白表达的相对量。结果显示，ＡｎｇⅡ刺激ＶＳＭＣｓ增生，其蛋白含量增加了４３６％（Ｐ＜００１），但不抑制ｐ２７蛋白的表达；ＡＶＰ可轻度抑制ｐ２７的表达，有轻度促进ＶＳＭＣｓ增殖和增生的作用（Ｐ＜００５）；ＰＤＧＦ明显抑制ｐ２７的表达，引起细胞增殖。本研究结果提示，ｐ２７蛋白抑制ＶＳＭＣｓ通过Ｇ１期进入Ｓ期，是抑制ＶＳＭＣｓ增殖的重要调节因子。
The p27 protein, a member of the cyclin-dependent kinase (Cdk) inhibitory protein family, reacts predominantly to external signals that promote or inhibit cell proliferation. In this study, flow cytometry (FCM) double labeling method was used to observe the effects of angiotensin Ⅱ (AngⅡ), vasopressin (AVP) and platelet derived growth factor (PDGF) on the cell cycle percentage and p27 The effect of protein expression. After adding AngⅡ, AVP and PDGFBB, VSMCs cultured in resting state were harvested at different time points and labeled with propidium iodide (PI) to determine the cell cycle. The cells were labeled with p27 protein monoclonal antibody and FITC-labeled secondary antibody, and the relative amount of p27 protein expression was determined by measuring the amount of fluorescence excited by flow cytometry. The results showed that AngⅡstimulated VSMCs proliferation, the protein content increased by 43.6% (P <001), but did not inhibit the expression of p27 protein; AVP could slightly inhibit the expression of p27, slightly promote VSMCs proliferation and proliferation (P <005); PDGF significantly inhibited the expression of p27, causing cell proliferation. Our results suggest that p27 inhibits the entry of VSMCs into S phase via G1 phase and is an important regulator of VSMCs proliferation.