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我们应用ＷｅｓｔｅｒｎＢｌｏｔ法、组织原位分子杂交、细胞生长曲线和ＤＮＡ合成等技术，检测９例人类乳腺癌细胞株和２０例乳腺癌标本中胰岛素样生长因子结合蛋白－３（ＩＧＦＢＰ－３）基因的蛋白质和ｍＲＮＡ水平的表达，研究其生物学作用。结果：①在乳腺癌细胞株中，雌激素受体阴性（ＥＲ－）者，分泌大量的ＩＧＦＢＰ－３，而雌激素受体阳性（ＥＲ＋）者不分泌ＩＧＦＢＰ－３；②在乳腺癌病人标本中，ＥＲ（－）者ＩＧＦＢＰ－３ｍＲＮＡ的定量表达明显高于ＥＲ（＋）者（Ｐ＜０．００５）；③ＩＧＦＢＰ－３能明显增强乳腺癌ＭＣＦ－７细胞株胰岛素样生长因子－Ⅰ（ＩＧＦ－Ⅰ）所致的细胞生长和ＤＮＡ合成作用。结论：我们认为ＩＧＦＢＰ－３基因蛋白质和ｍＲＮＡ的表达水平有可能成为乳腺癌患者的预后指标。
We used WesternBlot, tissue in situ hybridization, cell growth curves, and DNA synthesis to detect the expression of the insulin-like growth factor binding protein-3 (IGFBP-3) gene in 9 human breast cancer cell lines and 20 breast cancer specimens. Protein and mRNA levels are expressed and their biological effects are studied. Results: 1 Among the breast cancer cell lines, estrogen receptor negative (ER-) patients secreted large amounts of IGFBP-3, while estrogen receptor positive (ER+) patients did not secrete IGFBP-3; 2 breast cancer patients The expression of IGFBP-3 mRNA was significantly higher in ER(-) than in ER(+) (P<0.005); 3IGFBP-3 significantly enhanced the growth of breast cancer MCF-7 cell line insulin-like growth factor-I (IGF). -I) Cell growth and DNA synthesis. Conclusion: We believe that the expression level of IGFBP-3 protein and mRNA may be a prognostic indicator for breast cancer patients.