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本研究建立了一种新的PCR定量分析法——非同位素竞争性逆转录PCR法(Nonisotopic Compctitive RT-PCR Assay)测定MDR1基因的表达。 异硫氰酸胍法提取细胞总RNA,异硫氰酸胍CsCl法提取肿瘤组织细胞总RNA。竞争性RT-PCR的第一步为逆转录。将定量的细胞总RNA(1 μg)与
In this study, we established a new quantitative PCR method-Nonisotopic Compctitive RT-PCR Assay to detect the expression of MDR1 gene. The total RNA was extracted by guanidine isothiocyanate method and the total RNA was extracted by CsCl isothiocyanate method. The first step in competitive RT-PCR is reverse transcription. Quantitate total cellular RNA (1 μg) with