论文部分内容阅读
【目的】探讨清热活血调气方对坏死性小肠结肠炎(necrotizing enterocolitis,NEC)模型新生大鼠肠损伤的保护作用。【方法】选用SPF级3日龄Sprague-Daw ley新生大鼠36只,随机分为正常对照组、NEC模型组及治疗组,每组12只。NEC模型组采用鼠代乳品人工喂养、体积分数100%氮气缺氧90 s、4℃冷刺激10 min方法,每天2次,连续3 d,复制新生大鼠NEC模型;治疗组采用代乳品+清热活血调气方(30 mL.kg-1.次-1,3次/d)人工喂养,同时予缺氧冷刺激;正常对照组置于母鼠身旁喂养,不进行任何干预。各组于造模后第4天处死大鼠取出肠组织,取近回盲部肠组织做病理切片,观察组织学变化并做组织损伤评分,组织学评分≥2为NEC。【结果】与正常对照组比较,模型组肠损伤组织病理评分显著升高,体质量显著下降(P<0.01);与模型组比较,治疗组肠损伤组织病理评分与发病率显著降低,体质量显著升高(P<0.05或P<0.01)。【结论】清热活血调气方对NEC模型新生大鼠肠损伤具有一定保护作用。
【Objective】 To investigate the protective effects of “Qingre Huoxue Tiangong Prescription” on intestinal injury in neonatal rats with necrotizing enterocolitis (NEC). 【Methods】 Thirty-six newborn Sprague-Dawley rats aged 3 days were randomly divided into normal control group, NEC model group and treatment group, with 12 rats in each group. The NEC model group was fed with artificial milk substitutes, hypoxia for 90 s in 100% N2, 10 min cooling at 4 ℃ for 3 d, Huoxue Tiaogan Fang (30 mL.kg-1. Times-1, 3 times / d) artificial feeding, hypoxia cold stimulation; normal control group placed in the side of the mother rats fed without any intervention. The rats in each group were sacrificed on the fourth day after the model was established, and the intestinal tissue was removed for histopathological examination. Histological changes were observed and tissue damage scores were obtained. The histological score ≥2 was NEC. 【Results】 Compared with the normal control group, the pathological score of intestinal injury in model group increased significantly and the body weight decreased significantly (P <0.01). Compared with the model group, the histopathological score and incidence of intestinal injury decreased remarkably in the model group, Significantly increased (P <0.05 or P <0.01). 【Conclusion】 Qingre Huoxue Tiangi Recipe has a protective effect on intestinal injury in neonatal rats with NEC model.