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目的探讨肿瘤抑制基因p16蛋白表达与外阴鳞状细胞癌及其临床病理之间的关系。方法采用免疫组化SABC染色法检查36例外阴鳞状细胞癌组织及30例外阴良性组织中p16蛋白的表达,应用SPSS 17.0软件进行统计学处理分析。结果外阴鳞状细胞癌组织中p16蛋白的阳性表达率与外阴良性组织相比,差异有统计学意义(P<0.05)。而在不同的病理分级中,高分化组织(G1)与中低分化组织中(G2、G3)p16蛋白的阳性表达率差异有统计学意义(P<0.05)。外阴鳞状细胞癌组织中p16蛋白的表达与患者年龄、临床分期差异无统计学意义(P>0.05)。结论 p16蛋白在外阴鳞状细胞癌组织中有一定程度的表达异常,该蛋白的检测有助于判断疾病预后,指导临床治疗,并为进一步开展基因治疗提供依据。
Objective To investigate the relationship between the expression of tumor suppressor gene p16 and vulvar squamous cell carcinoma and its clinical pathology. Methods Immunohistochemical SABC staining was used to detect the expression of p16 protein in 36 cases of vulvar squamous cell carcinoma and 30 cases of vulvar benign tissues. SPSS 17.0 software was used for statistical analysis. Results The positive rate of p16 protein in vulvar squamous cell carcinoma was significantly higher than that in benign tissues of vulva (P <0.05). In different pathological grades, the positive expression rate of p16 protein in highly differentiated tissue (G1) and moderately poorly differentiated tissue (G2, G3) was significantly different (P <0.05). The expression of p16 protein in vulvar squamous cell carcinoma was not significantly different with age and clinical stage (P> 0.05). Conclusion The p16 protein is abnormally expressed in vulvar squamous cell carcinoma to a certain extent. The detection of this protein is helpful to judge the prognosis of the disease and guide the clinical treatment, and provide the basis for further gene therapy.