论文部分内容阅读
根据植原体16S rDNA保守区设计Cycling探针LST2probe及引物,建立了梨衰退植原体Cycleave实时荧光PCR检测方法。结果表明,探针LST2probe能特异的检测梨衰退植原体,供试同一组内不同亚组植原体及参试病原细菌均为阴性,检测灵敏度可达0.5 pg/μL。该Cycleave实时荧光PCR检测方法可用于梨衰退植原体的快速检测,并为其他有害生物鉴定提供借鉴依据。
According to the conserved region of 16S rDNA of the phytoplasma, the Cyclin probe LST2probe and primers were designed and the cycleave real-time fluorescence PCR detection method was established. The results showed that the probe LST2probe could detect pest - degrading pears specifically, and all of the subgroups of phytoplasmas and test pathogenic bacteria in the same group were negative. The detection sensitivity was 0.5 pg / μL. The Cycleave real-time fluorescence PCR detection method can be used for the rapid detection of pear fimbriae phytoplasmas and provide references for the identification of other pests.