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作者发展了一种迅速且直接分析夏氏疟原虫药物抗性的短期培养系统。实验使用四株夏氏疟原虫,其中AJ和AS为氯喹敏感株,AS3CQ和AS30CQ是经体内长期选择得到的AS抗氯喹株。当小鼠红细胞早期环状体感染率达20％—30％时,心脏采血,经CF11纤维素柱除去白细胞和血小板,用完全RPMI-1640培养液(含10％胎牛血清,50μg/ml庆大霉素,4.2ml/100ml5％碳酸氢钠溶液,并添加Hepes和次黄嘌呤)稀释至
The authors have developed a short-term culture system that rapidly and directly analyzes the resistance of Plasmodium Chilisi drugs. Four strains of Plasmodium malariae were used in the experiment, in which AJ and AS were chloroquine sensitive strains, and AS3CQ and AS30CQ were AS chloroquine resistant strains obtained by long-term in vivo selection. When the rate of early erythrocyte infection was 20% -30%, the heart blood was collected and the white blood cells and platelets were removed by CF11 cellulose column. The cells were washed with complete RPMI-1640 medium (containing 10% fetal bovine serum, 50μg / ml Gentamicin, 4.2 ml / 100 ml of 5% sodium bicarbonate solution and addition of Hepes and hypoxanthine) to