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目的:研究福尔马林致痛后大鼠脊髓和背根神经节(dorsal root ganglion,DRG)内降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的时空变化规律,为探讨CGRP在伤害性信息传递中的机制和作用提供实验依据。方法:选取健康成年正常SD大鼠54只随机分为生理盐水对照组和福尔马林实验组;实验组为右侧足底皮下给予0.1 mL 5%福尔马林后,分别存活15 min、30min、1 h、3 h、6 h、12h、24h和72h(n=6),免疫组化结合图像分析技术观测CGRP在脊髓腰段及L4~6DRG的表达变化。结果:正常DRG、脊髓前角和后角内CGRP有一定的基础表达。福尔马林致痛后3 h DRG的CGRP表达上升,12 h~24 h达峰值,72 h基本降低到正常;福尔马林致痛后6 h脊髓后角CGRP的表达上调,24 h达到高峰,72 h降低至正常;脊髓前角CGRP未见明显变化。结论:福尔马林致痛引起DRG和脊髓的CGRP的表达呈现一定的时空模式,可能是其参与伤害性信息传递的机制之一。
OBJECTIVE: To investigate the spatial and temporal changes of calcitonin gene-related peptide (CGRP) in rat spinal cord and dorsal root ganglion (DRG) induced by formalin, Provide experimental basis for the mechanism and function of harmful information transmission. Methods: Fifty-four healthy adult SD rats were randomly divided into normal saline control group and formalin test group. The experimental group were given 0.1 mL 5% formalin subcutaneously on the right plantar and survived for 15 min, 30min, 1h, 3h, 6h, 12h, 24h and 72h (n = 6). The expression of CGRP in the lumbar spinal cord and L4 ~ 6 DRG were observed by immunohistochemistry and image analysis. Results: There was some basic expression of CGRP in normal DRG, spinal cord anterior horn and posterior horn. At 3 h after formalin injection, the expression of CGRP in DRG increased from 12 h to 24 h and decreased to normal after 72 h. The expression of CGRP in spinal dorsal horn increased 6 h after formalin injection and reached a peak at 24 h Peak, 72 h reduced to normal; spinal cord anterior horn CGRP no significant change. CONCLUSION: The formalin-induced pain-induced CGRP expression in DRG and spinal cord presents a certain spatial and temporal pattern, which may be one of the mechanisms involved in nociceptive transmission.