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目的分析基孔肯雅热流行与诱蚊诱卵指数的关系,调查白纹伊蚊成幼虫感染基孔肯雅病毒状况。方法基孔肯雅热流行期间,通过诱蚊诱卵器和布雷图指数调查蚊虫密度和采集蚊虫,用实时荧光PCR和细胞分离2种方法对野外捕获的白纹伊蚊体内病毒进行检测。结果确认基孔肯雅热暴发流行后,启动包括应急灭蚊的综合控制措施1周后,疫情得到有效控制,布雷图指数和诱蚊诱卵指数下降到5以下;采集的蚊样品按照时间和地点分成27份进行病毒检测,成蚊标本都显示病毒阴性,有3份乙醇浸泡处理的蚊幼虫标本为可疑阳性,占总幼虫标本(24份)的12.5%。细胞培养分离病毒均为阴性。该社区共报告病例253例,应急控制在22d结束。结论基孔肯雅热暴发流行时,诱蚊诱卵器法作为应急灭蚊安全、有效、简便易行的评价方法,尤其在成蚊控制效果评价和捕获成蚊检测带病毒指数上有优势,流行期间白纹伊蚊对基孔肯雅病毒的感染率、传播率有待进一步研究。
Objective To analyze the relationship between chikungunya fever and mosquito-ovulation index and investigate the status of infection of chikungunya virus by adult Aedes albopictus larvae. Methods During the period of Chikungunya fever, mosquito density and mosquito population were surveyed by mosquito-ovitrap and Brett index, and the viruses in the wild were detected by real-time fluorescence PCR and cell separation. After confirming the epidemic of chikungunya outbreak after the chikungunya outburst, the epidemic situation was effectively controlled after the comprehensive control measures including emergency mosquito control were started and the Brettiler index and the mosquito-ovulating index dropped to less than 5. The collected mosquito samples were classified according to time and The virus was detected in all 27 mosquito samples, and 3 samples of mosquito larvae treated with ethanol were suspiciously positive, accounting for 12.5% of the total larval samples (24 samples). Virus isolation from cell culture was negative. The community reported a total of 253 cases of cases, emergency control at the end of 22d. Conclusions As the epidemic of Chikungunya outbreak, the mosquito-ovitrap method is safe, effective and easy to evaluate for mosquito control. Especially, it has advantages in the evaluation of the control effect of adult mosquitoes and the detection of the virus index of adult mosquitoes. In the epidemic Aedes albopictus infection rate of Chikungunya virus, the transmission rate needs further study.