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目的对引起跨区域食物中毒的斯坦利沙门菌分离株进行耐药性检测和分子分型分析,追溯并明确病因食品,为该菌防控工作提供科学依据。方法采用微量肉汤稀释法对15株斯坦利沙门菌进行18种抗生素敏感性试验,同时对菌株基因组DNA经限制性内切酶Xba I酶切后进行脉冲场凝胶电泳(PFGE)分子分型,并与数据库中其他沙门菌比较。结果此次疫情分离菌株中食品分离株和腹泻患者分离株血清型一致,耐药谱基本一致。经PFGE溯源分析后具有100%同源的PFGE带型,数据库中无相同带型的菌株。结论本起跨区域食物中毒是由斯坦利沙门菌污染的煮花生引起,分离菌株对红霉素耐药,对氯霉素处于中介,对其他抗生素敏感。
Objective To investigate the drug resistance and molecular typing analysis of Salmonella typhimurium isolates that cause food poisoning across the region and to trace and clarify the etiological food to provide a scientific basis for the prevention and control of the bacteria. Methods Fifteen strains of Salmonella Stanis were tested for their antibiotic susceptibility with broth microdilution method. Meanwhile, the genomic DNA of the strains was digested with restriction endonuclease Xba I and then subjected to PFGE molecular typing , And compared with other Salmonella in the database. Results The serotypes of the isolates of the isolates from the epidemic isolates and diarrhea patients were the same, and the drug resistance spectrum was basically the same. After PFGE traceability analysis with 100% homologous PFGE pattern, no identical strains in the database. Conclusions This cross-regional food poisoning was caused by salmon-contaminated boiled peanuts. The isolates were resistant to erythromycin and chloramphenicol intermediates, which were sensitive to other antibiotics.