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利用均匀分布于水稻 1 2条染色体上的 72个 RFL P标记与 8种限制性内切酶 (Eco R 、Eco R 、H ind 、 Dra 、 Sca 、Xba 、Bam H 和 Bgl )的不同组合 ,对化学诱变剂 MNU诱变产生的巨大胚、甜胚乳两个突变体及其原始品种金南风三者之间进行 RFL P分析。结果表明 :巨大胚突变体与金南风、甜胚乳突变体与金南风以及两突变体之间的多态性分别为 8.3%、5 .6 %和 1 2 .5 %。巨大胚和甜胚乳两突变体基因突变频率分别为 0 .0 2 2 7和 0 .0 0 70。根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变
Using 72 RFL P markers evenly distributed on 12 chromosomes of rice and 8 different restriction enzymes (Eco R, Eco R, H ind, Dra, Sca, Xba, Bam H and Bgl) RFLP analysis was performed between three mutants of giant embryos and sweet endosperm produced by chemical mutagenesis MNU mutagenesis and their original species of southern wind. The results showed that the polymorphisms between the giant embryo mutant and the kiwifruit, the sweet endosperm mutant and the kiwi and the two mutants were 8.3%, 5.6% and 12.5% respectively. Mutations in the giant embryo and sweet endosperm mutants were 0.227 and 0.707, respectively. Based on the number of restriction enzymes revealing the polymorphisms, the mutations that are generated can be mostly classified as point mutations