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目的 :探讨氯化锂 毛果芸香碱 (匹罗卡品 )致疒间大鼠早期大脑少突胶质前体细胞变化及意义。方法 :对雄性成年SD大鼠先后腹腔注射氯化锂、毛果芸香碱 ,制成癫疒间持续状态动物模型 ;用免疫荧光组织化学法检测疒间 性发作后早期大鼠大脑皮质和海马CA1区NG2和O4阳性细胞数量。结果 :和对照组相比 ,除癫疒间后 1d组外其余各组大鼠脑皮质内NG2和O4阳性细胞都有明显的增加 ;癫疒间后 1d组海马CA1区的阳性细胞数明显减少 ;癫疒间 后 7d组皮质和海马CA1区NG2和O4阳性细胞数最多。结论 :氯化锂 毛果芸香碱致疒间 大鼠早期大脑NG2和O4表达增加 ,少突胶质前体细胞增多 ,并且和观测时间相关。
Objective: To investigate the changes and significance of oligodendrocyte precursor cells in early brain of rats induced by lithium pilocarpine (pilocarpine). Methods: Adult male Sprague-Dawley rats were injected intraperitoneally with lithium chloride and pilocarpine to establish an animal model of persistent epilepsy. Immunofluorescence histochemical staining was used to detect the expression of NG2 and cAMP in the cerebral cortex and hippocampus CA1 of rats after intermittent seizures O4 positive cell number. Results: Compared with the control group, the positive cells of NG2 and O4 in the cerebral cortex of rats in other groups were significantly increased except 1d after epilepsy; the number of positive cells in hippocampal CA1 area was significantly decreased on the 1st day after epilepsy At 7 days after epilepsy, the number of NG2 and O4 positive cells in cortex and hippocampus CA1 area were the highest. CONCLUSION: The expression of NG2 and O4 in early brain and the oligodendrocyte precursors increased in lithium chloride pilocarine-induced rats and correlated with the observation time.