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在收集蘑菇、草菇等有菌膜的食用菌的孢子时,一般是选取菌膜将破未破的种菇,用升汞溶液浸泡消毒,再用无菌水冲洗,然后装进孢子收集器,给以一定的湿度和温度,并保持适当的透气性,进行培养、收集孢子。我们在实践中发现,这样的采孢方法有以下几点不足之处: (1)因种菇的成熟度较低,培养收集孢子的时间较长(2～3天),且往往不能充分开伞弹射孢子,或只能弹射少量孢子。对于草菇,种菇甚至未充分开伞弹射孢子即已自溶腐烂,造成采孢失败。 (2)种菇的菌膜很薄,在消毒和洗涤时容易破裂,致使消毒液或洗涤水渗入菌褶,杀死孢子或引入杂菌,从而影响孢子的质量。 (3)种菇采下后,营养来源断绝,孢
In the collection of mushrooms, mushrooms and other fungi membrane edible fungus spores, the general is to choose the membrane will break unbreakable mushrooms, soak disinfection with mercury solution, and then rinse with sterile water, and then into the spore collector , To give a certain humidity and temperature, and maintain proper breathability, culture, collection of spores. We found in practice, this method of sporefiltration has the following deficiencies: (1) Due to the low maturity of mushrooms, spores collected for a long time (2 to 3 days), and often can not fully open Umbrella bounces spores, or only a small amount of spores. For mushrooms, mushrooms do not even fully open the umbrella ejection spores that have been autolysis decay, resulting in spore failure. (2) the mushroom membrane is very thin, easy to rupture during disinfection and washing, resulting in disinfectant or wash water infiltration pleat, kill spores or the introduction of bacteria, thus affecting the quality of spores. (3) mining mushrooms, the source of nutrients cut off, spore