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目的:探讨HBV转录调控核心序列在肝细胞中转录的活性。方法:构建HBV增强子和启动子调控的荧光素酶报告载体,用脂质体转染法将其转染肝细胞和非肝细胞,双荧光素酶报告基因试验检测其在不同细胞中的转录活性。结果:HBV启动子在2.2.15细胞中转录活性最高,在其他肝细胞中的转录活性显著高于非肝细胞。结论:由HBV启动子构建的载体可能是一种新颖的有前景的靶向性肝癌细胞基因治疗载体。
Objective: To investigate the transcriptional activity of HBV transcriptional regulatory core sequence in hepatocytes. Methods: The luciferase reporter vector containing HBV enhancer and promoter was constructed and transfected into hepatocytes and non-hepatocytes by lipofection method. The dual luciferase reporter gene assay was used to detect the transcription in different cells active. Results: HBV promoter had the highest transcriptional activity in 2.2.15 cells and transcriptional activity in other hepatocytes was significantly higher than that in non-hepatocytes. CONCLUSION: The vector constructed by HBV promoter may be a novel and promising gene therapy vector for targeted hepatocellular carcinoma.