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目的建立同时测定补肾强身胶囊中淫羊藿苷、朝藿定C、特女贞苷的高效液相色谱(HPLC)法。方法采用SHIMADZUVP-ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速1.0 mL·min~(-1),柱温35℃,检测波长0~30 min为224 nm,30~45 min为270 nm。结果淫羊藿苷、朝藿定C、特女贞苷分别在3.55~177.70μg·mL~(-1)(r=0.999 9)、3.98~199.07μg·mL~(-1)(r=0.999 8)、3.49~174.97μg·mL~(-1)(r=0.999 9)浓度范围内线性关系良好。结论该方法可用于补肾强身胶囊的质量控制。
Objective To establish a high performance liquid chromatography (HPLC) method for simultaneous determination of icariin, epimedin C, and virginia in Bushen Qiangshen Capsule. Methods The mobile phase consisted of acetonitrile-water with a flow rate of 1.0 mL · min -1 using a SHIMADZUVP-ODS column (250 mm × 4.6 mm, 5 μm) with a column temperature of 35 ° C and a detection wavelength of 0 to 30 min 224 nm, 30 ~ 45 min to 270 nm. Results Icariin, epimedin C, and special virginin were in the range of 3.55 ~ 177.70μg · mL -1 (r = 0.999 9), 3.98 ~ 199.07μg · mL -1 (r = 0.999 8), 3.49 ~ 174.97μg · mL ~ (-1) (r = 0.999 9) concentration range of good linear relationship. Conclusion This method can be used for quality control of Bushen Qiong Capsule.