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硬粒小麦４２８６在灌浆期间，籽粒总氨基酸（ＴＡＡ）以籽粒干物质百分含量表示，呈“Ｖ”型变化，低谷出现在花后第２４－２８ｄ；籽粒中人体必需氨基酸（ＥＡＡ）呈平缓下降状。以千粒籽粒中绝对含量表示，ＴＡＡ和ＥＡＡ均呈“Ｓ”曲线增长。增施Ｎ肥可使ＴＡＡ的峰谷和ＥＡＡ下降幅度趋于平缓，说明增Ｎ不仅可以提高ＴＡＡ和ＥＡＡ的总量，还可提高积累强度，但ＥＡＡ／ＴＡＡ的比例不变，可见Ｎ肥很难改变蛋白质四组分之间的比例．增Ｎ可增加各种ＥＡＡ的含量，但增幅不同，氨基酸Ｍｅｔ和Ｐｌｚｅ增幅较大，三种施肥处理平均增幅在１５％以上，而氨基酸Ｌｅｎ、Ｉｌｅｕ的增幅较小。
During grain filling, the total amino acid (TAA) of Durum 4286 was expressed as a percentage of dry matter in kernel, which showed a “V” shape, and the lowest appeared at 24-28 days after anthesis. The essential amino acids (EAA) Fall like. In terms of the absolute content of one thousand grain, both TAA and EAA showed “S” curve growth. The increase of N fertilizer can make the decline of TAA peak and EAA to be gentle, indicating that increasing N can not only increase the total amount of TAA and EAA, but also increase the accumulation intensity, but the ratio of EAA / TAA remains unchanged Difficult to change the ratio between the four components of the protein. Increasing N increased the content of various EAA, but the increasing rate was different. The increase of amino acids Met and Plze was larger. The average increment of three kinds of fertilization treatments was above 15%, while the increase of amino acids Len and Ileu was small.