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目的：研究脑反复缺血后海马细胞外液氨基酸和单胺递质及其代谢产物的变化规律。方法：采用Ｐｕｌｓｉｎｅｌｌｉ和Ｂｒｉｅｒｌｅｙ ４血管闭塞的方法，使鼠脑反复缺血，海马微管透析及高压液相电化学检测，观察细胞外谷氨酸（Ｇｌｕ），天门冬氨酸（Ａｓｐ），谷氨酰胺，牛磺酸，丙氨酸，丝氨酸，多巴胺（ＤＡ），５－羟色胺（５－ＨＴ）及其代谢产物浓度的变化。结果：缺血期，Ｇｌｕ和Ａｓｐ骤然增高５０倍和３０倍。缺血期ＤＡ和５－ＨＴ含量分别增加３０倍和５０倍，随后逐渐下降，再灌注１００ｍｉｎ恢复到基线水平，与此同时，其酸性代谢产物３，４二羟苯乙酸（ＤＯＰＡＣ），高香草酸（ＨＶＡ），５－羟吲哚乙酸（５－ＨＩＡＡ）在缺血期明显下降。结论：缺血期海马细胞外液兴奋性氨基酸和单胺递质急剧大量释放并触发膜离子通道改变，Ｃａ２＋超载，自由基反应，共同介导神经元缺血死亡。
Objective: To study the changes of extracellular amino acids and monoamine neurotransmitters and their metabolites in hippocampus after repeated cerebral ischemia. Methods: Pulsinelli and Brierley 4 vessel occlusion were used to detect repeated ischemia, hippocampal microtubule dialysis and high-pressure liquid-phase electrochemical detection. The effects of extracellular glutamate (Glu), aspartate (Asp) Aminoamide, Taurine, Alanine, Serine, Dopamine (DA), Serotonin (5-HT) and its Metabolites. Results: In the ischemic period, Glu and Asp increased suddenly 50 times and 30 times. The content of DA and 5-HT in ischemic phase increased by 30 times and 50 times respectively, then decreased gradually and returned to the baseline level after 100 minutes of reperfusion. At the same time, its acid metabolites 3,4-Dophenylacetic acid (DOPAC) Acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) decreased significantly during ischemia. CONCLUSION: Excitatory amino acids and monoamine neurotransmitters in the hippocampus of hippocampus are rapidly released in large quantities and trigger changes of membrane ion channels, Ca2 + overload and free radical reaction, and jointly induce neuronal ischemic death.