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目的 观察葛根素对全脑缺血再灌流后脑组织诱导型一氧化氮合酶的影响。方法 将实验大鼠随机分为空白对照组、缺血再灌流盐水组及葛根素组 ,采用Pulsinelli4血管阻塞法造成全脑缺血再灌流模型 ,用免疫组织化学染色法行大鼠脑组织的iNOS测定。结果 随缺血再灌流时间的延长 ,盐水组iNOS逐渐降低 ,较空白对照组差异明显 (P <0 0 5 ) ,且再灌流时间越长 ,此差异越显著 (P<0 0 1) ;而葛根素组iNOS逐渐增高 ,缺血再灌流 2 4h时 ,葛根素组较盐水组iNOS明显增高 (P <0 0 5 ) ,接近空白对照组。结论 全脑缺血再灌流后 ,脑组织iNOS系统有明显损伤 ,葛根素可减轻这种损伤。
Objective To observe the effect of puerarin on inducible nitric oxide synthase in brain tissue after global cerebral ischemia and reperfusion. Methods Rats were randomly divided into control group, ischemia-reperfusion group and puerarin group. Pulsinelli4 vascular occlusion method was used to establish a global cerebral ischemia reperfusion model. Immunohistochemical staining was used to determine the iNOS activity in rat brain. Determination. Results With the prolongation of ischemic reperfusion time, iNOS in saline group gradually decreased, compared with the blank control group (P < 0.05), and the longer the reperfusion time, the more significant this difference (P <0 01); In the puerarin group, the iNOS level was gradually increased. After ischemia and reperfusion for 24 hours, the iNOS in the puerarin group was significantly higher than that in the saline group (P < 0.05), which was close to the blank control group. Conclusion After global cerebral ischemia and reperfusion, iNOS system in brain tissue is obviously damaged. Puerarin can alleviate this damage.