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采用化学共沉淀法制备由左旋多巴包裹的四氧化三铁磁性纳米粒子,合成磁性纳米粒子之后使用1-乙基-(3-二甲基氨基丙基)碳酰二亚胺盐酸盐(EDC)活化左旋多巴表面的羧基官能团,与特定的核酸适配子上的氨基发生脱水缩合反应,构建具有核酸适配子与左旋多巴修饰的磁性纳米粒子。通过X射线衍射(XRD)、红外检测(FT-IR)、粒径分析(SEM)、磁性能测定(VSM)等检测方法对磁性纳米粒子制备的各个阶段进行检测,并将改性磁性纳米粒子与内皮祖细胞(EPCs)共混培养,评价磁性纳米粒子的细胞相容性以及磁性纳米粒子与EPCs短时间的结合效果,并在外加磁场的作用下评价改性磁性纳米粒子对EPCs的定向引导效果。结果表明,制备的改性后磁性纳米粒子有良好的磁响应性,并且在一定的浓度范围内有较好的细胞相容性,短时间对EPCs具有较好的结合作用,初步实现了在体外动态环境下对EPCs的定向引导。
The magnetic iron oxide nanoparticles coated with levodopa were prepared by chemical coprecipitation. After magnetic nanoparticles were synthesized, 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride ( EDC) activates the carboxyl functional groups on the surface of levodopa and undergoes dehydration condensation reaction with the amino group on a specific aptamer to construct a magnetic nanoparticle with aptamer and levodopa modification. The preparation of magnetic nanoparticles was detected by X-ray diffraction (XRD), infrared detection (FT-IR), particle size analysis (SEM) and magnetic properties measurement (VSM). The modified magnetic nanoparticles Were cultured with endothelial progenitor cells (EPCs) to evaluate the cytocompatibility of magnetic nanoparticles and the short-term binding effect between magnetic nanoparticles and EPCs. The effect of modified magnetic nanoparticles on the directional guidance of EPCs was evaluated under the applied magnetic field effect. The results showed that the prepared modified magnetic nanoparticles have good magnetic responsiveness, good cell compatibility at a certain concentration range, good binding ability to EPCs in a short period of time, and initially achieved in vitro Directed EPCs under Dynamic Environment.