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以‘富士’苹果叶片为材料,采用刺伤接种法,比较了苹果树腐烂病菌强致病菌LXS080601和弱致病菌LXS081501侵染对寄主体内丙二醛(MDA)含量、渗透调节物质及防御酶活性的影响。结果表明,两菌株侵染后,叶片MDA、蛋白质和可溶性糖含量均增加,接种LXS080601叶片MDA含量快速上升,最大增幅为141.15%,而接种LXS081501叶片的MDA含量变化较小,增幅仅为1.16%~16.24%,但后者可溶性糖和蛋白质含量增幅分别高达158.12%和113.57%,显著高于接种LXS080601的处理。同时,两菌株均能诱导叶片内4种防御酶活性的升高,但LXS081501诱导的苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、过氧化氢酶(CAT)和多酚氧化酶(PPO)活性均显著高于LXS080601,说明不同致病力腐烂病菌对寄主防御酶活性的影响存在显著差异。
Fuji apple leaves were used as material to study the effects of the pathogen Camptotheca acuminata LXS080601 and the pathogen LXS081501 on the malondialdehyde (MDA) content, osmoregulation substances and defense Effect of enzyme activity. The results showed that the content of MDA, protein and soluble sugar in leaves increased after inoculation of two strains. The content of MDA in leaves of inoculated LXS080601 increased rapidly with the maximum increase of 141.15%. However, the content of MDA in leaves of inoculated LXS081501 changed only slightly by 1.16% ~ 16.24%, but the content of soluble sugar and protein in the latter increased by 158.12% and 113.57% respectively, which was significantly higher than that of LXS080601. At the same time, the two strains could induce the activity of four kinds of defense enzymes in leaves, but LXS081501 induced PAL, POD, CAT, The activity of phenoloxidase (PPO) was significantly higher than that of LXS080601, indicating that there was a significant difference in the effect of different pathogenic bacteria on host defense enzyme activity.