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[Objectives] To study changes in SOD,POD,CAT,MAD and Pro content of S.miltiorrhiza Bunge seedlings and the effects of brassinolide( BR) on drought resistance of S.miltiorrhiza Bunge. [Methods] 6 varieties of S.miltiorrhiza Bunge produced in Zhongjiang County of Sichuan Province were used as research object,and the method of PEG-6000 simulated drought stress was applied. [Results]20% PEG stress 9 d was used as the optimal simulation condition of drought stress. Under the same degree of drought stress,tissue-cultured big leaf S.miltiorrhiza Bunge showed the highest SOD and POD activity,tissue-cultured small leaf S.miltiorrhiza Bunge showed highest CAT activity,long-stalked big leaf S.miltiorrhiza Bunge had lower MDA content than other varieties,and Zhongjiang wild S.miltiorrhiza Bunge had the highest Pro content. [Conclusions] BR can indirectly improve drought resistance ability of S.miltiorrhiza Bunge,and 0.1mg/L BR has the best action effect.
[Objectives] To study changes in SOD, POD, CAT, MAD and Pro content of S. miltiorrhiza Bunge seedlings and the effects of brassinolide (BR) on drought resistance of S. miltiorrhiza Bunge. [Methods] 6 varieties of S. miltiorrhiza Bunge produced in Zhongjiang County of Sichuan Province were used as research object, and the method of PEG-6000 simulated drought stress was applied. [Results] 20% PEG stress 9 d was used as the optimal simulation condition of drought stress. Under the same degree of drought stress, tissue-cultured big leaf S.miltiorrhiza Bunge showed the highest SOD and POD activity, tissue-cultured small leaf S.miltiorrhiza Bungeained highest CAT activity, long-stalked big leaf S.miltiorrhiza Bunge had lower MDA content than other varieties, and Zhongjiang wild S.miltiorrhiza Bunge had the highest Pro content. [Conclusions] BR can indirectly improve drought resistance ability of S. miltiorrhiza Bunge, and 0.1 mg / L BR has the best action effect.