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目的为了研究中国庚型肝炎病毒（ＨＧＶ）非结构（ＮＳ３）区基因结构特征。方法利用逆转录半巢式聚合酶链反应从河南１份ＨＧＶＲＮＡ阳性血清获得覆盖ＨＧＶＮＳ３全长ｃＤＮＡ的４个片段，并克隆到ｐｃＤＮＡⅡ载体中，采用Ｓａｎｇｅｒ双脱氧末端终止法测定全部ｃＤＮＡ序列。结果发现克隆到的包括ＨＧＶＮＳ３区在内的ｃＤＮＡ序列长度为２１３７个核苷酸，编码７１１个氨基酸。与国内外已测定的５侏全序列的相应序列进行同源性比较，其核苷酸同源性在８５７％～９３８％之间，根据核苷酸序列推导的氨基酸序列的同源性为９６１％～９７３％，３株中国ＨＧＶＮＳ３区氨基酸存在３个共同变异位点，此外在河南株ＮＳ３区还发现了一个与其他５株全序列共同的糖基化位点。结论河南株ＨＧＶＮＳ３区的核苷酸及氨基酸序列相对比较保守，其基因特征的阐明为ＮＳ３区基因的生物学功能研究及ＨＧＶ诊断试剂的研制提供了基础。
Objective To study the structural characteristics of the non-structural (NS3) region of hepatitis G virus (HGV) in China. Methods Four fragments covering the full-length HGVNS3 cDNA were obtained from 1 HGV RNA-positive sera of Henan by reverse transcription-semi-nested-polymerase chain reaction and cloned into pcDNAⅡ vector. All the cDNA sequences were determined by Sanger dideoxy terminator method. As a result, the cloned cDNA sequence including the HGVNS3 region was found to be 2137 nucleotides in length, encoding 711 amino acids. Compared with the corresponding sequences of the 5 Juvenile sequences in China and other countries, the nucleotide homologies were between 857% and 938%, and the amino acid sequences deduced from nucleotide sequences There were three common mutation sites in the three HGVNS3 regions of China, and a common glycosylation site was found in the NS3 region of Henan strain with the other five full sequences . Conclusion The nucleotide and amino acid sequences of HGVNS3 region in Henan are relatively conservative. The elucidation of the gene characteristics provides the basis for the biological function of NS3 gene and the development of HGV diagnostic reagent.