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目的:建立准确可靠的测定大鼠血浆中20(R)-人参皂苷Rh2的液相色谱-质谱联用(LC/MS)方法,研究20(R)-Rh2在大鼠不同肠段中的吸收动力学.方法:分离、结扎SD大鼠的不同肠段(十二指肠、空肠、回肠、结肠),制成在体肠袢模型,将20(R).Rh2按剂量9 mg/kg注入肠袢,于不同时间点眼内眦取血,用LC/MS法测定给药后的血浆中药物浓度,计算吸收动力学参数.结果:本文所建立的大鼠血浆中20(R).Rh2的LC/MS法线性范围为5～1000 ng/mL(R~2=0.9973),日内日间精密度在15%之内,准确度在85%～115%之间.十二指肠、空肠、回肠和结肠肠袢给药后的AUG_(0-6h)分别为(207±88)、(301±49)、(157±93)和(172±68)ng·mL~(-1)·h,吸收速率常数K_a分别为(3.9±0.4)、(1.6±0.4)、(1.9±0.8)和(1.1±0.9)/h,达峰时间t_(max)分别为(0.44±0.13)、(1.75±0.50)、(1.13±0.63)和(2.00±1.41)h,达峰浓度分别为(82±17)、(110±11)、(36±8)和(43±7)ng/mL.结论:20(R)-Rh2肠道吸收困难,十二指肠和空肠是它吸收的主要部位.推测膜通透性差和肠道外排转运体是影响Rh2吸收的因素.“,”AIM: To establish a precise and reliable LC/MS assay for determining 20(R)-Ginsenoside Rh2 in rat plasma and to study the absorption kinetics of 20 (R)-Ginsenoside Rh2 in different rat intestinal segments. METHODS: Different rat intestinal segments (duodenum, jejunum, ileum and colon) were isolated and ligated respectively, to form a closed intestinal loop model. 20(R)-Rh2 was injected into the loop directly at a dose of 9 mg/kg. The blood was harvested at different time points after dosage. Concentrations of 20(R)-Ginsenoside Rh2 in plasma were determined by the LC/MS method. The parameters were calculated. RESULTS: 20(R)-Rh2 had a good linearity from 5 to 1000 ng/mL(R~2 = 0.9973) in the LC/MS method established in this paper. The within-day and inter-day precisions were within 15%, and the accuracy was between 85% - 115% . After administering in the intestinal loops, the AUC_(0-6h) were (207 ±88), (301 ±49), (157 ±93) and (172±68) ng·mL~(-1)·h for duodenum, jejunum, ileum and colon respectively, while the absorption rate constant K_a were (3.9 + 0.4), (1.6 ±0.4), (1.9 ±0.8) and (1.1 ±0.9) /h, respectively. The peak time t_(max) were(0.44 ± 0.13), (1.75 ± 0.50), (1.13 ± 0.63) and (2.00 ± 1.41) respectively, with the corresponding peak concentrations at (82±17), (110±ll), (36±8)and (43 ±7) ng/mL. CONCLUSION: It is hard for 20(R)-Rh2 to be absorbed in rat intestine. Duodenum and jejunum are its main absorption sites. It is speculated that bad membrane permeability and intestinal efflux transporters are impact factors for its absorption.