【摘 要】
：
Severe influenza infections are often associated with the excessive induction of pro-inflammatory cytokines,which is also referred to as “cytokine storms”.Several studies have shown that cytokine storms are directly associated with influenzainduced fatal
【机 构】
：
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 43
论文部分内容阅读
Severe influenza infections are often associated with the excessive induction of pro-inflammatory cytokines,which is also referred to as “cytokine storms”.Several studies have shown that cytokine storms are directly associated with influenzainduced fatal acute lung injury and acute respiratory distress syndrome.Due to the narrow administration window,current antiviral therapies are often inadequate.The efforts to use immunomodulatory agents alone or in combination with antiviral agents in the treatment of influenza in animal models have resulted in the achievement of protective effects accompanied with reduced cytokine production.Currently,there are no immunomodulatory drugs for influenza available for clinical use.Animal models,despite being ideal to study the anti-inflammatory responses to influenza virus infection,are very costly and time-consuming.Therefore,there is an urgent need to establish fast and economical screening methods using cellbased models to screen and develop novel immunomodulatory agents.In this study,we screened seven human cell lines and found that the human monocytic cell U937 supports the replication of different subtypes of influenza viruses as well as the production of the important pro-inflammatory cytokines and was selected to develop the cell-based model.The U937 cell model was validated by testing a panel of known antiviral and immunomodulatory agents and screening a drug library consisting of 1280 compounds comprised mostly of FDA-approved drugs.We demonstrated that the U937 cell model is robust and suitable for the high-throughput screening of immunomodulators and antivirals against influenza infection.
其他文献
Foot-and-mouth disease virus (FMDV) can infect domestic and wild cloven-hoofed animals.The non-structural protein 3D plays an important role in FMDV replication and pathogenesis.However,the interaction partners of 3D,and the effects of those interactions
To date,29 distinct microRNAs (miRNAs) have been reported to be expressed during herpes simplex virus infections.Sequence analysis of mature herpes simplex virus-1 (HSV-1) miRNAs revealed five sets of miRNAs that are complementary to each other:miR-H6-5p/
As the open reading frames of hepatitis B virus (HBV) genomes are overlapping,resistance mutations (MTs) in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type (WT) HB
The study was conducted to explore the mechanisms of sex differences in the response to chronic hepatitis B (CHB) in terms of DNA methylation,SNP genotype,and gene expression.Genomic DNA was isolated from peripheral blood mononuclear cells (PBMCs) of CHB
Human immunodeficiency virus-1 capsid (HIV-1 CA) is involved in different stages of the viral replication cycle.During virion assembly,CA drives the formation of the hexameric lattice in immature viral particles,while in mature virions CA monomers assembl
Currently there is no effective antiviral therapy for SARS-CoV-2 infection,which frequently leads to fatal inflammatory responses and acute lung injury.Here,we discuss the various mechanisms of SARS-CoV-mediated inflammation.We also assume that SARS-CoV-2
The incidence of Japanese encephalitis (JE) has significantly decreased in China due to JE vaccines.In this study,we investigated the post-JE vaccination seroprevalence and protection provided by vaccinated sera against Japanese encephalitis virus (JEV) t
Echovirus 18 (E18),a serotype of Enterovirus B (EV-B) species,is an important pathogen in aseptic meningitis.El8 had rarely been detected in mainland China,but became the predominant pathogen associated with viral encephalitis (VE) and meningitis in Hebei
Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis.Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential.In t
Early etiological diagnosis is very important for the control of sudden viral infections,and requires antibodies with both high sensitivity and high specificity.Traditional antibody preparation methods have limitations,such as a long and arduous cycle,com