Isolation, cultivation and identification of brain glioma stem cells by magnetic bead sorting

• 作 者: Xiuping Zhou Chao Zheng Qiong
• 机 构: DepartmentofNeurosurgery,LaboratoryofNeurosurgery,JiangsuKeyLaboratoryofBrainDiseaseBiology,Graduate
• 出 处: 中国神经再生研究：英文版
• 发表日期: 2012年13期
• 关键词: 脑胶质瘤 分离培养 免疫磁珠 干细胞 鉴定 胶质纤维酸性蛋白 无血清培养基 微管相关蛋白 brain glioma stem cells CD133 nes
• 基金项目: supported in part by the National Natural Science Foundation of China,No.81072072,31070933,,the guidance project of Xuzhou Science and Technology Bureau,No.X22D1056

This study describes a detailed process for obtaining brain glioma stem cells from freshly dissected human brain glioma samples using an immunomagnetic bead technique combined with serum-free media pressure screening.Furthermore,the proliferation,differen