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培养两种不同分化程度的人胃腺癌ＳＧＣ－７９０１和ＮＫＭ－４５细胞，分离其胞浆和胞核ＫＣＩ抽提液。用葡聚糖包被活性炭饱和分析法分别测定各细胞组分中雌激素受体（ＥＲ）、孕激素受体（ＰＲ）、雄激素受体（ＡＲ）。结果：ＳＧＣ－７９０１细胞胞浆胞核抽提液中ＥＲ含量为３０．２和３５．７ｆｍｏｌ／ｍｇ（１ｆｍｏｌ＝１０－１５ｍｏｌ）蛋白，ＰＲ含量为２０．３和２２．７ｆｍｏｌ／ｍｇ蛋白，ＮＫＭ－４５细胞胞浆和胞核抽提液中ＥＲ含量为２４．８和２５．１ｆｍｏｌ／ｍｇ蛋白，ＰＲ含量为１６．４和１８．６ｆｍｏｌ／ｍｇ蛋白。而两种细胞ＡＲ均为阴性（＜６～１０ｆｍｏｌ／ｍｇ蛋白）。结果说明两种胃癌细胞均为ＥＲ、ＰＲ阳性，而分化程度更低、转移性较强的ＳＧＣ－７９０１细胞株ＥＲ、ＰＲ含量较高。提示ＥＲ水平与胃癌细胞的分化程度相关，ＥＲ阳性胃癌组织似为性激素依赖性，内分泌治疗可能有效果。
Two different degrees of differentiation of human gastric adenocarcinoma SGC-7901 and NKM-45 cells were cultured, and the cytosol and nucleus KCI extracts were isolated. The estrogen receptor (ER), progesterone receptor (PR) and androgen receptor (AR) were measured in each cell fraction by a dextran coated activated carbon saturation assay. RESULTS: The ER content in cytoplasmic nucleus extracts of SGC-7901 cells was 30.2 and 35.7 fmol/mg (1 fmol=10-15 mol) protein, PR content was 20.3 and 22.7 fmol/mg protein, NKM The ER content in cytosol and nucleus extracts of -45 cells was 24.8 and 25.1 fmol/mg protein, PR content was 16.4 and 18.6 fmol/mg protein. Both cell ARs were negative (<6 to 10 fmol/mg protein). The results showed that the two gastric cancer cells were positive for ER and PR, while the ER and PR levels were lower in the SGC-7901 cell line with lower differentiation and stronger metastasis. It is suggested that ER levels are related to the degree of differentiation of gastric cancer cells. ER-positive gastric cancer tissues seem to be sex hormone-dependent, and endocrine therapy may have an effect.