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目的筛选出衰老大鼠睾丸组织受何首乌饮调控的差异表达基因。方法根据实验要求将雄性大鼠分为4组,每组各10只:青年对照组(12月龄);自然衰老组(18月龄);何首乌饮1组(何首乌饮4.8g/100g,灌胃60d,18月龄);何首乌饮2组(何首乌饮4.8g/100g,灌胃30d,18月龄),采用基因芯片技术筛选睾丸组织受何首乌饮调控的差异表达基因,实时荧光定量PCR(qRT-PCR)进行验证。结果基因芯片共筛选出差异表达基因21260个,自然衰老组和青年对照组大鼠睾丸组织差异表达基因有2100个,其中受何首乌饮直接调控的基因有912个,其中衰老后691个基因表达明显上调,221个基因表达下调,用何首乌饮干预60d后,上调的691个基因明显下调,下调的221个基因明显上调;通过GO分析睾丸组织受何首乌饮调控的基因参与的生物学过程有繁殖,免疫代谢,细胞生长发育死亡等;Pathway分析,这些基因主要分布在59条通路上;经qRT-PCR检测结果与芯片结果一致。结论何首乌饮延缓大鼠睾丸组织衰老是一个多途径、多靶点协同作用的过程,但其具体作用机制尚不明确,有待进一步研究。
Objective To screen differentially expressed genes that regulate the testicular tissue of senile rats induced by Hepou Wuyin. Methods According to the experimental requirements, male rats were divided into 4 groups with 10 rats in each group: young control group (12 months old); natural aging group (18 months old); Polygonum multiflorum group 1 (Polygonum multiflorum 4.8g / 100g, (Polygonum multiflorum drink 4.8g / 100g, gavage 30d, 18months), using gene chips to screen the differentially expressed genes regulated by He Shouyin in the testis tissue, real-time quantitative PCR qRT-PCR). Results A total of 21260 differentially expressed genes were screened by gene chip. There were 2100 differentially expressed genes in testes of natural aging group and young control group, of which 912 were directly regulated by He Shou Wu Yin, of which 691 genes were significantly expressed after aging 221 genes were down-regulated, and 691 genes were down-regulated and down-regulated 221 genes were significantly up-regulated after 60 days of treatment with Polygonum multiflorum.While the biological processes involved in the regulation of testicular tissue by Polygonum hydropiper, Immune metabolism, cell growth and development death; Pathway analysis, these genes are mainly distributed in 59 pathways; by qRT-PCR test results and chip results. Conclusion Polygonum multiflorum Thunb drink can delay the senescence of testis in rats, which is a multi-path and multi-target synergistic process. However, its specific mechanism of action is not yet clear and remains to be further studied.