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目的通过线粒体DNA测序初步确定新疆乌恰县多房棘球蚴的来源。方法新疆维吾尔自治区南疆乌恰县泡球蚴病患者5例,采集其病灶组织,提取DNA,PCR扩增多房棘球绦虫线粒体DNA Nad2序列,并与已发表的泡球蚴线粒体DNA Nad2序列进行序列比对和系统进化分析。结果新疆南疆乌恰县是一个新的泡型包虫病流行区。Nad2基因序列比对分析及遗传距离矩阵显示,5例AE患者病灶扩增的Em Nad2基因存在差异,人体感染的虫株之间存在低的遗传变异。Nad2基因系统进化树分析表明,这些虫株是亚洲多房棘球绦虫亚型,包含3个单倍体型。结论新疆乌恰县多房棘球绦虫流行株属于亚洲基因型,至少有3种单倍体型。关于乌恰县多房棘球绦虫终末宿主和中间宿主的种群以及其在疾病的传播中的作用需要进一步证实。
Objective To determine the origin of Echinococcus multilocularis in Wuqia County, Xinjiang by using mitochondrial DNA sequencing. Methods Five cases of alveolar neoplasms in Wuqia County, southern Xinjiang Uygur Autonomous Region were collected and their DNAs were collected. The mitochondrial DNA Nad2 sequence of Echinococcus multilocularis was amplified by PCR and compared with the published sequence of Nad2 Sequence alignments and phylogenetic analyzes were performed. Results Wuqia County, southern Xinjiang is a new bubonic hydatid disease endemic area. Sequence alignment analysis of Nad2 gene and genetic distance matrix showed that there were differences in the amplification of Em Nad2 gene in 5 AE patients and low genetic variation among human infected strains. Phylogenetic tree analysis of Nad2 gene indicated that these strains were subtype of Echinococcus multilocularis in Asia and consisted of three haplotypes. Conclusion The epidemic strains of Echinococcus multilocularis in Wuqia County of Xinjiang belong to Asian genotypes, with at least 3 haplotypes. The population of terminal and intermediate hosts of Echinococcus multilocularis in Wuqia County and their role in disease transmission needs further confirmation.